Abstract
Micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in micropropagated plants is the first step towards ensuring genetic uniformity in mass production of planting material. In the present study, 31 plants of guava cultivar “Lucknow 49” regenerated by micropropagation were tested for genetic fidelity by comparing them to the mother plant from which explant material was obtained. Efficient rooting of in vitro proliferated shoots was obtained by culture on 1/2 strength MS medium supplemented with either 9.8 μM indole butyric acid (IBA) or 11.4 μM indole acetic acid (IAA). Leaf samples of 31 regenerated plants were compared to the mother plant using 17 simple sequence repeat (SSR) markers. While 16 SSRs detected the same allele, locus mPgCIR07 detected slight differences, where six micropropagated plants were 1 bp smaller (152 bp) than the parental genotype (153 bp). Differences in leaf tissues for anthocyanin pigmentation were also noted among micropropagated plants. Results of the study indicated efficient rooting of “Lucknow-49” cultivar for rapid propagation of planting material, and revealed that micropropagated plants were identical for 16 of the 17 loci examined. Although most mutations induced by tissue culture may not have an effect on phenotype, the possibility that novel phenotypes can be generated in a commercial setting exists.
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Claims 5
Claim · model-written summary
IBA supported efficient rooting of micropropagated guava shoots.
- Taxon
- Psidium guajava L.
Psidium guajavat0 - Treatment
- 9.8 μM indole butyric acid (IBA)e0
- Outcome
- Efficient rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Psidium guajava L.”
e0“Efficient rooting of in vitro proliferated shoots was obtained by culture on 1/2 strength MS medium supplemented with either 9.8 μM indole butyric acid (IBA) or 11.4 μM indole acetic acid (IAA)”
Claim · model-written summary
IAA supported efficient rooting of micropropagated guava shoots.
- Taxon
- Psidium guajava L.Psidium guajavat0
- Treatment
- 11.4 μM indole acetic acid (IAA)e0
- Outcome
- Efficient rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Psidium guajava L.”
e0“Efficient rooting of in vitro proliferated shoots was obtained by culture on 1/2 strength MS medium supplemented with either 9.8 μM indole butyric acid (IBA) or 11.4 μM indole acetic acid (IAA)”
Claim · model-written summary
Sixteen SSR loci had the same allele in regenerated and mother plants.
- Taxon
- Psidium guajava L.Psidium guajavat0
- Outcome
- 16 SSRs detected the same allelee0
- Value
- 16 SSRse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Psidium guajava L.”
e0“While 16 SSRs detected the same allele, locus mPgCIR07 detected slight differences”
Claim · model-written summary
At mPgCIR07, six regenerated plants had a 152 bp allele, 1 bp smaller than the 153 bp parental allele.
- Taxon
- Psidium guajava L.Psidium guajavat0
- Gene
- mPgCIR07e0
- Outcome
- six micropropagated plants were 1 bp smallere0
- Value
- 152 bpe0
- Qualifier
- parental genotype (153 bp)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Psidium guajava L.”
e0“locus mPgCIR07 detected slight differences, where six micropropagated plants were 1 bp smaller (152 bp) than the parental genotype (153 bp)”
Claim · model-written summary
Leaf anthocyanin pigmentation differed among micropropagated plants.
- Taxon
- Psidium guajava L.Psidium guajavat0
- Organ / stage
- leaf tissuese0
- Outcome
- Differences in leaf tissues for anthocyanin pigmentatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Psidium guajava L.”
e0“Differences in leaf tissues for anthocyanin pigmentation were also noted among micropropagated plants”
The same questions in other papers
indole-3-butyric acid · growth · Psidium guajava (guava)Results differ
35 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
34 papers more in this group
See all 38 papers →indole-3-acetic acid · growth · Psidium guajava (guava)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.