Abstract
Abstract. The main goal of the present study was to isolate DNA both experimental and commercial wines in order to differentiate some Romanian grapevine cultivars (Tămâioasă Românescă, Galbenă de Odobeşti, Fetească Neagră and Busuioacă de Bohotin) by PCR using wine as a sample. Extracted residual grape DNA quality and specificity were verified using co-dominant simple sequence repeat (SSR) markers (VVS2, VVMD27, VVMD5 and VVMD7). The results obtained in this research shows that even 18 months after wine fermentation could be detected residual DNA that can be suitable for DNA fingerprinting. The 12-month-old experimental wine samples were more amplified than the 18-month-old ones. Among the microsatellite tested, VVS2 and VVMD7 gave satisfactory results. The VVS2 marker, which generated the shortest PCR amplicons (119-138 bp), amplified all of the cultivars analyzed in the wines samples. In case of commercial wines authentication of grape material by PCR using wine as a biological material was unsuccessfully due to insufficient amount of residual DNA in samples analyzed.
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Claims 6
Claim · model-written summary
Residual grape DNA could be detected in wine up to 18 months after fermentation and was suitable for DNA fingerprinting.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“even 18 months after wine fermentation could be detected residual DNA that can be suitable for DNA fingerprinting”
Claim · model-written summary
Experimental 12-month-old wine samples were more amplified than the 18-month-old ones.
- Treatment
- 12-month-old experimental wine samplese2
- Outcome
- amplifiede2
- Direction
- moree2
- Comparator
- 18-month-old onese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“The 12-month-old experimental wine samples were more amplified than the 18-month-old ones”
Claim · model-written summary
Among the microsatellites tested, VVS2 and VVMD7 gave satisfactory results.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“Among the microsatellite tested, VVS2 and VVMD7 gave satisfactory results”
Claim · model-written summary
The VVS2 marker generated the shortest PCR amplicons, of 119-138 bp.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“The VVS2 marker, which generated the shortest PCR amplicons (119-138 bp), amplified all of the cultivars analyzed in the wines samples”
Claim · model-written summary
The VVS2 marker amplified all of the grapevine cultivars analyzed in the wine samples.
- Outcome
- amplified all of the cultivarse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“The VVS2 marker, which generated the shortest PCR amplicons (119-138 bp), amplified all of the cultivars analyzed in the wines samples”
Claim · model-written summary
Authentication of commercial wine grape material by PCR was unsuccessful due to insufficient residual DNA.
Verbatim
e1“differentiate some Romanian grapevine cultivars”
e2“In case of commercial wines authentication of grape material by PCR using wine as a biological material was unsuccessfully due to insufficient amount of residual DNA in samples analyzed”