Abstract
Soybean microsomes incubated with or without 1 {mu}M 2,4-D loose phosphatidylcholine, phosphatidylinositol and other phospholipids. Vesicles labeled in vivo with ({sup 14}C)choline prior to isolation and incubation exhibit rapid 2,4-D-induced hydrolysis of phosphatidylcholine to a plateau within 15 min. As much as 50% of the radioactivity of the membrane appeared in the soluble supernatant. However, when separated into plasma membrane (PM)-enriched and PM-depleted fractions, the PM-enriched fraction was unresponsive to auxin. Similarly, highly purified pM fractions from soybean microsomes exhibited no auxin-stimulated breakdown of phosphoinosotides, and no stimulations of phospholipase C or of phospholipase A{sub 2}. Instead, PM exhibited an auxin-stimulated NADH oxidase activity not shown by microsomes depleted of PM. The results suggest that in vitro auxin effects on phospholipid metabolism involve components of the microsomal fraction other than PM such as the endoplasmic reticulum or tonoplast, whereas PM responds by enhanced NADH oxidase activity.
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Claims 7
Claim · model-written summary
In soybean microsomes, 2,4-D induced rapid hydrolysis of phosphatidylcholine that reached a plateau within 15 minutes, compared with incubation without 2,4-D.
- Taxon
- Soybean
Glycine maxe0 - Treatment
- 2,4-De0
- Outcome
- hydrolysis of phosphatidylcholinee6
- Direction
- increaseinferrede6
- Comparator
- without 1 {mu}M 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean microsomes incubated with or without 1 {mu}M 2,4-D loose phosphatidylcholine”
e6“exhibit rapid 2,4-D-induced hydrolysis of phosphatidylcholine to a plateau within 15 min”
Claim · model-written summary
As much as 50% of membrane radioactivity appeared in the soluble supernatant after 2,4-D treatment of soybean microsomes.
- Taxon
- SoybeanGlycine maxe0
- Treatment
- 2,4-De0
- Outcome
- radioactivity of the membrane appeared in the soluble supernatante7
- Direction
- increaseinferrede7
- Value
- 50%e7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean microsomes incubated with or without 1 {mu}M 2,4-D loose phosphatidylcholine”
e7“As much as 50% of the radioactivity of the membrane appeared in the soluble supernatant”
Claim · model-written summary
The PM-enriched fraction of soybean microsomes was unresponsive to auxin.
- Taxon
- SoybeanGlycine maxe0
- Organ / stage
- PM-enriched fractione1
- Treatment
- auxine1
- Outcome
- unresponsive to auxine1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean microsomes incubated with or without 1 {mu}M 2,4-D loose phosphatidylcholine”
e1“the PM-enriched fraction was unresponsive to auxin”
Claim · model-written summary
Highly purified plasma-membrane fractions of soybean microsomes showed no auxin-stimulated breakdown of phosphoinositides.
- Taxon
- soybeanGlycine maxe2
- Treatment
- auxine2
- Outcome
- breakdown of phosphoinosotidese2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“highly purified pM fractions from soybean microsomes exhibited no auxin-stimulated breakdown of phosphoinosotides”
Claim · model-written summary
Highly purified plasma-membrane fractions of soybean microsomes showed no auxin-stimulated phospholipase C stimulation.
- Taxon
- soybeanGlycine maxe2
- Treatment
- auxine2
- Outcome
- stimulations of phospholipase Ce3
- Direction
- no_changeinferrede3
- Qualifier
- or of phospholipase A{sub 2}e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“highly purified pM fractions from soybean microsomes exhibited no auxin-stimulated breakdown of phosphoinosotides”
e3“no stimulations of phospholipase C or of phospholipase A{sub 2}”
Claim · model-written summary
The plasma-membrane fraction of soybean microsomes showed auxin-stimulated NADH oxidase activity that microsomes depleted of plasma membrane did not show.
- Taxon
- soybeanGlycine maxe2
- Organ / stage
- PMe4
- Treatment
- auxine4
- Outcome
- NADH oxidase activitye4
- Direction
- stimulatede4
- Comparator
- microsomes depleted of PMe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“highly purified pM fractions from soybean microsomes exhibited no auxin-stimulated breakdown of phosphoinosotides”
e4“PM exhibited an auxin-stimulated NADH oxidase activity not shown by microsomes depleted of PM”
Claim · model-written summary
Auxin effects on phospholipid metabolism in vitro appear to involve microsomal components other than the plasma membrane, such as the endoplasmic reticulum or tonoplast.
- Taxon
- soybeanGlycine maxe2
- Organ / stage
- endoplasmic reticulum or tonoplaste5
- Treatment
- auxine5
- Outcome
- phospholipid metabolisme5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“highly purified pM fractions from soybean microsomes exhibited no auxin-stimulated breakdown of phosphoinosotides”
e5“in vitro auxin effects on phospholipid metabolism involve components of the microsomal fraction other than PM such as the endoplasmic reticulum or tonoplast”
The same questions in other papers
class · physiology: hormones · Glycine max (soybean)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.