Abstract
Agranal thylakoid membranes from leaves of Phaseolus vulgaris L. were fragmented using seven distinct detergents: digitonin, Triton X-100, cetylpyridinium chloride, sodium dodecylsulfate, and Zwittergents 3-12, 3-14, and 3-16, differing in chemical composition and/or electric charges. Subchloroplast particles separated on a Percoll gradient were examined by flow cytometry to determine their size and shape. Vesicle size was also determined by a haematological analyzer, which produced comparable results. Individual green bands consisted of vesicles of fairly wide size distribution. Simple direct proportionality between the particle density and their size was not observed in any case, nevertheless, bigger particles were more abundant in fractions of higher density. Some vesicles had even a larger size than the original thylakoids. This might reflect a specific action of the detergents in low concentrations on agranal membranes, with incorporation of detergent molecules into vesicles. Inner structures of particles of the same size and density were not necessarily identical, but represented several populations, as was apparent from the side scatter analysis. Flow cytometric analysis can thus be used for the investigation of mechanisms of membrane fragmentation by detergents.
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Claims 7
Claim · model-written summary
The haematological analyzer produced comparable vesicle size results.
- Taxon
- Phaseolus vulgaris L.
Phaseolus vulgarist0 - Outcome
- Vesicle sizee0
- Comparator
- comparable resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vesicle size was also determined by a haematological analyzer, which produced comparable results.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Green bands contained vesicles with a wide size distribution.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- vesicles of fairly wide size distributione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Individual green bands consisted of vesicles of fairly wide size distribution.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Particle density and size were not directly proportional.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- direct proportionality between the particle density and their sizee0
- Direction
- not observede0
- Comparator
- particle density and their sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simple direct proportionality between the particle density and their size was not observed in any case, nevertheless, bigger particles were more abundant in fractions of higher density.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Bigger particles were more abundant in higher density fractions.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- bigger particlese0
- Direction
- more abundante0
- Setting
- fractions of higher densitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simple direct proportionality between the particle density and their size was not observed in any case, nevertheless, bigger particles were more abundant in fractions of higher density.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Some vesicles exceeded the original thylakoids in size.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- sizee0
- Direction
- largere0
- Comparator
- original thylakoidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some vesicles had even a larger size than the original thylakoids.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Particles of the same size and density contained several inner structural populations.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- Inner structures of particles of the same size and densitye0
- Direction
- not necessarily identicale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inner structures of particles of the same size and density were not necessarily identical, but represented several populations, as was apparent from the side scatter analysis.”
t0“leaves of Phaseolus vulgaris L.”
Claim · model-written summary
Flow cytometry was proposed for investigating detergent-driven membrane fragmentation.
- Taxon
- Phaseolus vulgaris L.Phaseolus vulgarist0
- Outcome
- investigation of mechanisms of membrane fragmentation by detergentse0
- Comparator
- detergentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Flow cytometric analysis can thus be used for the investigation of mechanisms of membrane fragmentation by detergents.”
t0“leaves of Phaseolus vulgaris L.”