Abstract
Several isolates from a newly described group of fast-growing acid-producing soybean rhizobia, Rhizobium japonicum, were analyzed for plasmid content. All contained from one to four plasmids with molecular weights of 100 x 10 or larger. Although most of the isolates shared plasmids of similar size, the restriction endonuclease (BamHI, EcoRI, and HindIII) patterns of the plasmids from three of the isolates were vastly different. Growth in the presence of acridine orange was effective in producing mutants cured of the largest plasmid in one of the strains. These mutants also lost the ability to form nodules on soybeans. High-temperature curing of a smaller plasmid in another strain did not lead to loss of nodulating ability or alteration of symbiotic effectiveness on soybean cultivars. The identities of all of the isolates and mutants were ascertained by immunofluoresence and immunodiffusion. The new fast-growing strains of R. japonicum may provide a better genetic system for the study of the soybean symbiosis than the slow-growing R. japonicum, not all of which can be shown to contain plasmids.
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Claims 7
Claim · model-written summary
The soybean rhizobial isolates contained one to four large plasmids.
- Taxon
- Soybeans
Glycine maxt0 - Interactor
- Rhizobium japonicume1symbiont
- Outcome
- plasmidse0
- Value
- one to four plasmidse0
- Qualifier
- molecular weights of 100 x 10 or largere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All contained from one to four plasmids with molecular weights of 100 x 10 or larger.”
t0“Soybeans”
e1“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
Three rhizobial isolates had very different plasmid restriction patterns despite similar plasmid sizes in most isolates.
- Taxon
- SoybeansGlycine maxt0
- Interactor
- Rhizobium japonicume1symbiont
- Outcome
- restriction endonuclease (BamHI, EcoRI, and HindIII) patternse0
- Direction
- vastly differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although most of the isolates shared plasmids of similar size, the restriction endonuclease (BamHI, EcoRI, and HindIII) patterns of the plasmids from three of the isolates were vastly different.”
t0“Soybeans”
e1“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
Acridine orange treatment produced rhizobial mutants cured of the largest plasmid.
- Taxon
- SoybeansGlycine maxt0
- Treatment
- Growth in the presence of acridine orangee0
- Interactor
- Rhizobium japonicume1symbiont
- Outcome
- mutants cured of the largest plasmide0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth in the presence of acridine orange was effective in producing mutants cured of the largest plasmid in one of the strains.”
t0“Soybeans”
e1“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
Mutants cured of the largest plasmid lost the ability to nodulate soybeans.
- Taxon
- soybeansGlycine maxe0
- Treatment
- mutants cured of the largest plasmide1
- Interactor
- Rhizobium japonicume2symbiont
- Outcome
- ability to form nodulese0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These mutants also lost the ability to form nodules on soybeans.”
e1“mutants cured of the largest plasmid in one of the strains”
e2“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
Curing a smaller rhizobial plasmid at high temperature did not remove nodulation ability.
- Taxon
- soybeanGlycine maxe0
- Treatment
- High-temperature curing of a smaller plasmide0
- Interactor
- Rhizobium japonicume1symbiont
- Outcome
- nodulating abilitye0
- Direction
- did not lead to losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-temperature curing of a smaller plasmid in another strain did not lead to loss of nodulating ability or alteration of symbiotic effectiveness on soybean cultivars.”
e1“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
Curing a smaller rhizobial plasmid at high temperature did not alter symbiotic effectiveness on soybean cultivars.
- Taxon
- soybeanGlycine maxe0
- Treatment
- High-temperature curing of a smaller plasmide0
- Interactor
- Rhizobium japonicume1symbiont
- Outcome
- symbiotic effectivenesse0
- Direction
- did not lead to loss of nodulating ability or alteratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-temperature curing of a smaller plasmid in another strain did not lead to loss of nodulating ability or alteration of symbiotic effectiveness on soybean cultivars.”
e1“soybean rhizobia, Rhizobium japonicum”
Claim · model-written summary
The authors suggest that fast-growing R. japonicum strains may offer a better genetic system for studying soybean symbiosis.
- Taxon
- soybeanGlycine maxe0
- Interactor
- R. japonicume0symbiont
- Outcome
- better genetic system for the study of the soybean symbiosise0
- Comparator
- slow-growing R. japonicume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new fast-growing strains of R. japonicum may provide a better genetic system for the study of the soybean symbiosis than the slow-growing R. japonicum, not all of which can be shown to contain plasmids.”
The same questions in other papers
heat · symbiosis · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.