Abstract
Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here, we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most down-regulated, with almost a three-fold reduction in infected samples. This down-regulation led to a concomitant up-regulation of some of their targets, such as Squamosa promoter-binding protein-like 9 and 13, as well as Scarecrow-like 6. The processing of miRNA precursors, pre-miR156 and pre-miR171, in sweet orange seems to be affected by the virus. For instance, virus infection increases the level of unprocessed precursors, which is accompanied by a concomitant decrease in mature species accumulation. miR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171a suggests that the alteration in the processing of these precursors might be caused by a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and the CPsV 24K protein. This study contributes to the understanding of the manner in which a virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.
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Claims 10
Claim · model-written summary
Citrus psorosis virus infection alters the accumulation of a set of endogenous miRNAs in sweet orange.
- Taxon
- Sweet orange
Citrus sinensise0 - Interactor
- Citrus psorosis virusCitrus psorosis viruse1pathogen
- Outcome
- accumulation of a set of endogenous microRNAs (miRNAs)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e1“the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs)”
Claim · model-written summary
miR156, miR167 and miR171 were the most down-regulated miRNAs in infected samples, with almost a three-fold reduction.
- Taxon
- Sweet orangeCitrus sinensise0
- Treatment
- infected samplese2
- Interactor
- Citrus psorosis virusCitrus psorosis viruse1pathogen
- Outcome
- miR156, miR167 and miR171e2
- Direction
- down-regulatede2
- Value
- almost a three-fold reductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e1“the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs)”
e2“Within these miRNAs, miR156, miR167 and miR171 were the most down-regulated, with almost a three-fold reduction in infected samples”
Claim · model-written summary
Down-regulation of miRNAs led to up-regulation of some of their targets, including Squamosa promoter-binding protein-like 9 and 13 and Scarecrow-like 6.
- Taxon
- Sweet orangeCitrus sinensise0
- Gene
- Squamosa promoter-binding protein-like 9 and 13, as well as Scarecrow-like 6e3
- Outcome
- some of their targetse3
- Direction
- up-regulatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e3“This down-regulation led to a concomitant up-regulation of some of their targets, such as Squamosa promoter-binding protein-like 9 and 13, as well as Scarecrow-like 6”
Claim · model-written summary
Virus infection increased the level of unprocessed miRNA precursors.
- Taxon
- Sweet orangeCitrus sinensise0
- Treatment
- virus infectione4
- Interactor
- viruse4pathogen
- Gene
- pre-miR156 and pre-miR171e4
- Outcome
- level of unprocessed precursorse4
- Direction
- increasese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e4“The processing of miRNA precursors, pre-miR156 and pre-miR171, in sweet orange seems to be affected by the virus. For instance, virus infection increases the level of unprocessed precursors, which is accompanied by a concomitant decrease in mature species accumulation”
Claim · model-written summary
Virus infection decreased the accumulation of mature miRNA species.
- Taxon
- Sweet orangeCitrus sinensise0
- Treatment
- virus infectione4
- Interactor
- viruse4pathogen
- Outcome
- mature species accumulatione4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e4“The processing of miRNA precursors, pre-miR156 and pre-miR171, in sweet orange seems to be affected by the virus. For instance, virus infection increases the level of unprocessed precursors, which is accompanied by a concomitant decrease in mature species accumulation”
Claim · model-written summary
miR156a primary transcript accumulation remained unaltered.
- Taxon
- Sweet orangeCitrus sinensise0
- Outcome
- miR156a primary transcript accumulatione5
- Direction
- unalterede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e5“miR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript”
Claim · model-written summary
miR156a processing is suggested to be deregulated.
- Taxon
- Sweet orangeCitrus sinensise0
- Gene
- miR156ae5
- Outcome
- processing deregulatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e5“miR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript”
Claim · model-written summary
The viral 24K protein may cause altered processing of pre-miR156a and pre-miR171a through direct or indirect interaction.
- Taxon
- Sweet orangeCitrus sinensise0
- Interactor
- viral 24K proteine6pathogen
- Outcome
- alteration in the processing of these precursorse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e6“The co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171a suggests that the alteration in the processing of these precursors might be caused by a direct or indirect interaction with this particular viral protein”
Claim · model-written summary
The viral 24K protein co-immunoprecipitated with pre-miR156a or pre-miR171a.
- Taxon
- Sweet orangeCitrus sinensise0
- Interactor
- viral 24K proteine7pathogen
- Outcome
- co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171ae7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e7“The co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171a”
Claim · model-written summary
Both miRNA precursors and the CPsV 24K protein are nuclear localized.
- Taxon
- Sweet orangeCitrus sinensise0
- Interactor
- CPsVe1pathogen
- Outcome
- nuclear localization of both miRNA precursors and the CPsV 24K proteine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange (Citrus sinensis)”
e1“This result is also consistent with the nuclear localization of both miRNA precursors and the CPsV 24K protein”
The same questions in other papers
infection · molecular: metabolites · Citrus sinensis (sweet orange)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
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