Abstract
Leaf extracts of 14-d-old pea (Pisum sativum L. cv Homesteader) seedlings were examined for folate derivatives and for 10-formyltetrahydrofolate synthetase (SYN), 5,10-methenyltetrahydrofolate cyclohydrolase (CYC), and 5,10-methylenetetrahydrofolate dehydrogenase (DHY) activities. Microbiological and enzyme assays showed that leaf folates SYN, CYC, and DHY were predominantly cytosolic. Extracts of Percoll gradient-purified mitochondria contained less than 1% of total leaf folate and less that 1% of each enzyme activity. Fractionation of whole-leaf homogenates resulted in the copurification of DHY and CYC (subunit 38 kD) and the isolation of a SYN protein (subunit 66 kD). Polyclonal antibodies were raised against purified cytosolic DHY-CYC (DHY-CYC-Ab) and cytosolic SYN (SYN-Ab), respectively. Immunoblots showed that DHY-CYC-Ab cross-reacted with a mitochondrial protein band (38 kD). Two mitochondrial protein bands (subunit Mr = 40,000 and 44,000) cross-reacted with SYN-Ab. Immunoaffinity chromatography (DHY-CYC-Ab as the immobile ligand) indicated that the bulk of mitochondrial SYN activity was not associated with mitochondrial DHY or CYC. When 9-d-old etiolated pea seedlings were exposed to light for up to 3 d, the specific enzyme activities of DHY-CYC in whole-leaf extracts rose 2-fold and more DHY-CYC-Ab cross-reacting protein was detected. In contrast, the specific activity of SYN fell from 5 to 1 [mu]mol min-1 mg-1 protein and less SYN-Ab cross-reacting protein was detected. The data suggest that in pea leaves, the bulk of one-carbon-substituted tetrahydrofolates and enzymes for the generation of 10-formyltetrahydrofolate are extra-mitochondrial.
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Claims 14
Claim · model-written summary
Leaf folates and SYN, CYC, DHY activities were predominantly cytosolic.
- Taxon
- pea
Lathyrus oleraceust0 - Outcome
- leaf folates SYN, CYC, and DHY were predominantly cytosolice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Microbiological and enzyme assays showed that leaf folates SYN, CYC, and DHY were predominantly cytosolic.”
t0“Pea Leaves”
Claim · model-written summary
Purified mitochondria had less than 1% of total leaf folate.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- total leaf folatee0
- Value
- less than 1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extracts of Percoll gradient-purified mitochondria contained less than 1% of total leaf folate and less that 1% of each enzyme activity.”
t0“Pea Leaves”
Claim · model-written summary
Purified mitochondria had less than 1% of each enzyme activity.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- each enzyme activitye0
- Value
- less that 1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extracts of Percoll gradient-purified mitochondria contained less than 1% of total leaf folate and less that 1% of each enzyme activity.”
t0“Pea Leaves”
Claim · model-written summary
Whole leaf fractionation copurified DHY and CYC with 38 kD subunits.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- DHY and CYCe0
- Outcome
- copurification of DHY and CYCe0
- Value
- 38 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fractionation of whole-leaf homogenates resulted in the copurification of DHY and CYC (subunit 38 kD) and the isolation of a SYN protein (subunit 66 kD).”
t0“Pea Leaves”
Claim · model-written summary
Whole leaf fractionation isolated SYN protein with a 66 kD subunit.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- SYNe0
- Outcome
- isolation of a SYN proteine0
- Value
- 66 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fractionation of whole-leaf homogenates resulted in the copurification of DHY and CYC (subunit 38 kD) and the isolation of a SYN protein (subunit 66 kD).”
t0“Pea Leaves”
Claim · model-written summary
DHY CYC antibody cross reacted with a mitochondrial 38 kD band.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- cross-reacted with a mitochondrial protein bande0
- Value
- 38 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoblots showed that DHY-CYC-Ab cross-reacted with a mitochondrial protein band (38 kD).”
t0“Pea Leaves”
Claim · model-written summary
SYN antibody cross reacted with a mitochondrial 40,000 Mr band.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- mitochondrial protein bandse0
- Value
- 40,000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two mitochondrial protein bands (subunit Mr = 40,000 and 44,000) cross-reacted with SYN-Ab.”
t0“Pea Leaves”
Claim · model-written summary
SYN antibody cross reacted with a mitochondrial 44,000 Mr band.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- mitochondrial protein bandse0
- Value
- 44,000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two mitochondrial protein bands (subunit Mr = 40,000 and 44,000) cross-reacted with SYN-Ab.”
t0“Pea Leaves”
Claim · model-written summary
Most mitochondrial SYN activity was not associated with mitochondrial DHY or CYC.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- bulk of mitochondrial SYN activity was not associated with mitochondrial DHY or CYCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoaffinity chromatography (DHY-CYC-Ab as the immobile ligand) indicated that the bulk of mitochondrial SYN activity was not associated with mitochondrial DHY or CYC.”
t0“Pea Leaves”
Claim · model-written summary
Light exposure doubled DHY CYC specific activity in etiolated pea seedlings.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- exposed to light for up to 3 de0
- Outcome
- specific enzyme activities of DHY-CYCe0
- Direction
- rosee0
- Value
- 2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When 9-d-old etiolated pea seedlings were exposed to light for up to 3 d, the specific enzyme activities of DHY-CYC in whole-leaf extracts rose 2-fold and more DHY-CYC-Ab cross-reacting protein was detected.”
Claim · model-written summary
Light exposure increased DHY CYC antibody cross reacting protein.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- exposed to light for up to 3 de0
- Outcome
- DHY-CYC-Ab cross-reacting proteine0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When 9-d-old etiolated pea seedlings were exposed to light for up to 3 d, the specific enzyme activities of DHY-CYC in whole-leaf extracts rose 2-fold and more DHY-CYC-Ab cross-reacting protein was detected.”
Claim · model-written summary
SYN specific activity fell from 5 to 1 micromol per minute per milligram protein.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- specific activity of SYNe0
- Direction
- felle0
- Value
- 5 to 1 [mu]mol min-1 mg-1 proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast, the specific activity of SYN fell from 5 to 1 [mu]mol min-1 mg-1 protein and less SYN-Ab cross-reacting protein was detected.”
t0“Pea Leaves”
Claim · model-written summary
Less SYN antibody cross reacting protein was detected after light exposure.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- SYN-Ab cross-reacting proteine0
- Direction
- lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast, the specific activity of SYN fell from 5 to 1 [mu]mol min-1 mg-1 protein and less SYN-Ab cross-reacting protein was detected.”
t0“Pea Leaves”
Claim · model-written summary
Authors infer that most one carbon substituted tetrahydrofolates and related enzymes occur outside pea mitochondria.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- bulk of one-carbon-substituted tetrahydrofolates and enzymes for the generation of 10-formyltetrahydrofolate are extra-mitochondriale0
- Qualifier
- suggeste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data suggest that in pea leaves, the bulk of one-carbon-substituted tetrahydrofolates and enzymes for the generation of 10-formyltetrahydrofolate are extra-mitochondrial.”
The same questions in other papers
light · soil: biology · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.