Abstract
The combination of low temperature EPR, magnetic circular dichroism, and resonance Raman spectroscopies reveals the presence of a single [3Fe-4S]+,0 center as the sole iron-sulfur prosthetic group in glutamate synthase from spinach leaves. The electronic, magnetic, and structural properties of the oxidized and reduced cluster are analogous with those of similar clusters in bacterial ferredoxins. It was not possible to convert the [3Fe-4S] cluster to a [4Fe-4S] cluster by incubating with iron under reducing conditions. Taken together with the published amino acid sequence data for plant and bacterial glutamate synthases, this suggests that the [3Fe-4S] cluster is not an isolation artifact resulting from oxidative degradation of a [4Fe-4S] cluster. The likelihood that a [3Fe-4S] cluster is an intrinsic component of all plant and bacterial glutamate synthases is discussed.
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Claims 4
Claim · model-written summary
Spinach glutamate synthase has one [3Fe-4S]+,0 center as its sole iron-sulfur group.
- Taxon
- spinach
Spinacia oleraceat0 - Gene
- glutamate synthasee0
- Outcome
- a single [3Fe-4S]+,0 center as the sole iron-sulfur prosthetic groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“spinach”
e0“a single [3Fe-4S]+,0 center as the sole iron-sulfur prosthetic group in glutamate synthase from spinach leaves”
Claim · model-written summary
Oxidized and reduced cluster properties resemble bacterial ferredoxin clusters.
- Taxon
- spinachSpinacia oleraceat0
- Outcome
- electronic, magnetic, and structural propertiese0
- Comparator
- similar clusters in bacterial ferredoxinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“spinach”
e0“The electronic, magnetic, and structural properties of the oxidized and reduced cluster are analogous with those of similar clusters in bacterial ferredoxins”
Claim · model-written summary
Incubation with iron under reducing conditions did not convert the [3Fe-4S] cluster to [4Fe-4S].
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- incubating with iron under reducing conditionse0
- Outcome
- convert the [3Fe-4S] cluster to a [4Fe-4S] clustere0
- Direction
- not possiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“spinach”
e0“It was not possible to convert the [3Fe-4S] cluster to a [4Fe-4S] cluster by incubating with iron under reducing conditions”
Claim · model-written summary
The authors infer that the [3Fe-4S] cluster is not an isolation artifact.
- Taxon
- spinachSpinacia oleraceat0
- Outcome
- [3Fe-4S] cluster is not an isolation artifacte0
- Qualifier
- oxidative degradation of a [4Fe-4S] clustere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“spinach”
e0“this suggests that the [3Fe-4S] cluster is not an isolation artifact resulting from oxidative degradation of a [4Fe-4S] cluster”
The same questions in other papers
iron atom · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results
5 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.