Abstract
In vitro mutation method was used to obtain salt-tolerant clone in apricot. Small propagules of Canino apricot cultivar were irradiated with gamma ray at doses of 0, 10, 25, 35, 50, 75 and 100 Gy. After 30 days from treatment, both the radio sensitivity and post-irradiation recovery were assessed as the number of proliferated shoots per explants, fresh weight of cultures, shoot length and productivity of irradiated explants. A sudden and sharp decrease in the survival percentage occurred with the dose 75 Gy, while the highest dose (100 Gy) was lethal for all propagules. A marked decline in the number of regenerated shoots per explant and fresh weight of produced cultures was associated with an increase of irradiation doses. Doses in the range of 10-75 Gy, which preserved high survival percentage of irradiated explants, seemed to be more suitable for in vitro mutation in Canino apricot cultivar. Irradiated shoots were exposed to different concentrations of NaCl which were added to the multiplication medium at the rates of 25, 50, 75, 100, 125 mM and after 30 days, vigorous shoots were selected from salinity treatments. In conclusion, apricot tissues exposed to different doses of gamma irradiation in the range of 10-75 Gy, followed by culturing the plantlets produced in a medium containing additional salts (ranging from 25 to 125 mM) can be considered a good method to identify the most tolerant mutants to salts in apricot cultivars.
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Claims 6
Claim · model-written summary
Survival percentage decreased sharply at 75 Gy.
- Taxon
- apricot
Prunus armeniacat0 - Treatment
- 75 Gye0
- Outcome
- survival percentagee0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A sudden and sharp decrease in the survival percentage occurred with the dose 75 Gy”
t0“Canino apricot cultivar”
Claim · model-written summary
The 100 Gy dose was lethal for all propagules.
- Taxon
- apricotPrunus armeniacat0
- Treatment
- 100 Gye0
- Outcome
- lethal for all propagulese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest dose (100 Gy) was lethal for all propagules.”
t0“Canino apricot cultivar”
Claim · model-written summary
Regenerated shoot number declined as irradiation dose increased.
- Taxon
- apricotPrunus armeniacat0
- Treatment
- irradiation dosese0
- Outcome
- number of regenerated shoots per explante0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A marked decline in the number of regenerated shoots per explant and fresh weight of produced cultures was associated with an increase of irradiation doses.”
t0“Canino apricot cultivar”
Claim · model-written summary
Culture fresh weight declined as irradiation dose increased.
- Taxon
- apricotPrunus armeniacat0
- Treatment
- irradiation dosese0
- Outcome
- fresh weight of produced culturese0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A marked decline in the number of regenerated shoots per explant and fresh weight of produced cultures was associated with an increase of irradiation doses.”
t0“Canino apricot cultivar”
Claim · model-written summary
Doses of 10-75 Gy appeared suitable for mutation because explant survival remained high.
- Taxon
- apricotPrunus armeniacae0
- Treatment
- 10-75 Gye0
- Outcome
- more suitable for in vitro mutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Doses in the range of 10-75 Gy, which preserved high survival percentage of irradiated explants, seemed to be more suitable for in vitro mutation in Canino apricot cultivar.”
Claim · model-written summary
Irradiation followed by saline culture was proposed to identify salt-tolerant mutants.
- Taxon
- apricotPrunus armeniacae0
- Treatment
- gamma irradiation in the range of 10-75 Gye0
- Outcome
- identify the most tolerant mutants to saltse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apricot tissues exposed to different doses of gamma irradiation in the range of 10-75 Gy, followed by culturing the plantlets produced in a medium containing additional salts (ranging from 25 to 125 mM) can be considered a good method to identify the most tolerant mutants to salts in apricot cultivars.”