Abstract
Ascorbate is oxidized to the free radical monodehydroascorbate by O2.- and by H2O2 (through the action of ascorbate peroxidase) formed in the Mehler reaction by isolated spinach (Spinacia oleracea) thylakoids. Light-dependent electron transport from water to monodehydroascorbate is shown to be coupled to ATP formation with a ratio ATP:O2 of 2. In the presence of ascorbate the net O2 exchange balance of the Mehler reaction is close to zero, and the synthesis of ATP is increased 2 to 3 times due to the extra electron transport to the monodehydroascorbate free radical. A scheme of the electron transport in the presence of ascorbate is discussed.
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Claims 4
Claim · model-written summary
Ascorbate was oxidized to monodehydroascorbate by reactive oxygen species from isolated spinach thylakoids.
- Taxon
- spinach
Spinacia oleraceae0 - Organ / stage
- thylakoidse0
- Gene
- ascorbate peroxidasee0
- Outcome
- Ascorbate is oxidized to the free radical monodehydroascorbatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ascorbate is oxidized to the free radical monodehydroascorbate by O2.- and by H2O2 (through the action of ascorbate peroxidase) formed in the Mehler reaction by isolated spinach (Spinacia oleracea) thylakoids.”
Claim · model-written summary
Electron transport to monodehydroascorbate was coupled to ATP formation at ATP:O2 of 2.
- Taxon
- spinachSpinacia oleraceae1
- Outcome
- ATP formatione0
- Value
- ATP:O2 of 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light-dependent electron transport from water to monodehydroascorbate is shown to be coupled to ATP formation with a ratio ATP:O2 of 2.”
t0“Photosynthetic Phosphorylation”
e1“isolated spinach (Spinacia oleracea) thylakoids”
Claim · model-written summary
Ascorbate made net oxygen exchange in the Mehler reaction close to zero.
- Taxon
- spinachSpinacia oleraceae1
- Treatment
- ascorbatee0
- Outcome
- net O2 exchange balancee0
- Direction
- close to zeroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of ascorbate the net O2 exchange balance of the Mehler reaction is close to zero”
t0“Photosynthetic Phosphorylation”
e1“isolated spinach (Spinacia oleracea) thylakoids”
Claim · model-written summary
Ascorbate increased ATP synthesis two to threefold.
- Taxon
- spinachSpinacia oleraceae1
- Treatment
- ascorbatee0
- Outcome
- synthesis of ATPe0
- Direction
- increasede0
- Value
- 2 to 3 timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the synthesis of ATP is increased 2 to 3 times due to the extra electron transport to the monodehydroascorbate free radical”
t0“Ascorbic Acid”
e1“isolated spinach (Spinacia oleracea) thylakoids”
The same questions in other papers
ascorbate · molecular: metabolites · Spinacia oleracea (spinach)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.