Abstract
Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25-46% as compared to immature ovaries (excised from flowers with microspores at a mid-late uninucleate stage). The highest numbers of embryos and green plants were produced when using mature Caramba ovaries. Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries. Moreover, Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%. Attempts were made to identify molecular mechanisms associated to the inductive effect of the ovaries on microspore embryogenesis. The genes TAA1b, FLA26, and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture. The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary. Our results open opportunities for new approaches to increase bread wheat doubled haploid production by anther culture, and to identify the functional components of the ovary inductive effect on microspore embryogenesis.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 19
Claim · model-written summary
Ovary pre-conditioned medium and ovary co-culture increased green doubled haploid plant production efficiency.
- Taxon
- bread wheat
Triticum aestivume0 - Treatment
- Ovary pre-conditioned medium and ovary co-culturee0
- Outcome
- efficiency of green doubled haploid plant productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture.”
Claim · model-written summary
The medium increased embryo numbers sixfold.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- this mediume0
- Outcome
- numbers of embryose0
- Direction
- increasee0
- Value
- 6- and 11-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively”
t0“bread wheat”
Claim · model-written summary
The medium increased green plant numbers elevenfold.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- this mediume0
- Outcome
- green plantse0
- Direction
- increasee0
- Value
- 11-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively”
t0“bread wheat”
Claim · model-written summary
The medium had a greater effect on a medium-low responding cultivar.
- Taxon
- bread wheatTriticum aestivumt0
- Outcome
- greater effect on a medium-low responding cultivare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“having a greater effect on a medium-low responding cultivar”
t0“bread wheat”
Claim · model-written summary
Ovary genotype and developmental stage significantly affected microspore embryogenesis.
- Taxon
- bread wheatTriticum aestivumt0
- Organ / stage
- developmental stagee0
- Outcome
- microspore embryogenesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovary genotype and developmental stage significantly affected microspore embryogenesis.”
t0“bread wheat”
Claim · model-written summary
Caramba ovaries doubled embryo numbers.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Carambae0
- Treatment
- Caramba ovariese0
- Outcome
- number of embryose0
- Direction
- increasee0
- Value
- 2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism.”
t0“bread wheat”
Claim · model-written summary
Caramba ovaries doubled green plant numbers.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Carambae0
- Treatment
- Caramba ovariese0
- Outcome
- green plantse0
- Direction
- increasee0
- Value
- 2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism.”
t0“bread wheat”
Claim · model-written summary
Caramba ovaries reduced albinism.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Carambae0
- Treatment
- Caramba ovariese0
- Outcome
- rate of albinisme0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism.”
t0“bread wheat”
Claim · model-written summary
Mature ovaries raised embryo numbers by 25–46% relative to immature ovaries.
- Taxon
- bread wheatTriticum aestivumt0
- Organ / stage
- late binucleate stagee0
- Treatment
- Mature ovariese0
- Outcome
- number of embryose0
- Direction
- raisede0
- Value
- 25-46%e0
- Comparator
- immature ovariese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25-46% as compared to immature ovaries”
t0“bread wheat”
Claim · model-written summary
Mature ovaries raised green plant numbers by 25–46% relative to immature ovaries.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- Mature ovariese0
- Outcome
- green plantse0
- Direction
- raisede0
- Value
- 25-46%e0
- Comparator
- immature ovariese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25-46% as compared to immature ovaries”
t0“bread wheat”
Claim · model-written summary
Mature Caramba ovaries produced the highest embryo and green plant numbers.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Carambae0
- Organ / stage
- maturee0
- Outcome
- numbers of embryos and green plantse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest numbers of embryos and green plants were produced when using mature Caramba ovaries.”
t0“bread wheat”
Claim · model-written summary
Galeón ovaries induced microspore embryogenesis at the same rate as Caramba ovaries.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Galeóne0
- Treatment
- Ovaries from Galeóne0
- Outcome
- microspore embryogenesise0
- Direction
- same ratee0
- Comparator
- Caramba ovariese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries.”
t0“bread wheat”
Claim · model-written summary
Tigre ovaries induced microspore embryogenesis at the same rate as Caramba ovaries.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Tigree0
- Treatment
- Tigree0
- Outcome
- microspore embryogenesise0
- Direction
- same ratee0
- Comparator
- Caramba ovariese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries.”
t0“bread wheat”
Claim · model-written summary
Kilopondio ovaries induced microspore embryogenesis at the same rate as Caramba ovaries.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Kilopondioe0
- Treatment
- Kilopondioe0
- Outcome
- microspore embryogenesise0
- Direction
- same ratee0
- Comparator
- Caramba ovariese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries.”
t0“bread wheat”
Claim · model-written summary
Tigre ovaries raised spontaneous chromosome doubling to 71%.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- Tigree0
- Treatment
- Tigre ovariese0
- Outcome
- percentage of spontaneous chromosome doublinge0
- Direction
- raisede0
- Value
- 71%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%.”
t0“bread wheat”
Claim · model-written summary
TAA1b, FLA26 and WALI6 were expressed or induced at different rates during ovary culture.
- Taxon
- wheatTriticum aestivume0
- Gene
- TAA1b, FLA26, and WALI6e0
- Outcome
- expressed and/or induced at different rates during ovary culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genes TAA1b, FLA26, and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture.”
Claim · model-written summary
CGL1 was expressed or induced at different rates during ovary culture.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- CGL1e0
- Outcome
- expressed and/or induced at different rates during ovary culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture.”
t0“bread wheat”
Claim · model-written summary
FER was expressed or induced at different rates during ovary culture.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- FERe0
- Outcome
- expressed and/or induced at different rates during ovary culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture.”
t0“bread wheat”
Claim · model-written summary
FLA26 and FER expression patterns could relate to genotype and stage differences in ovary induction.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- FLA26 and FERe0
- Outcome
- differences between genotypes and developmental stages in the inductive effect of the ovarye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary.”
t0“bread wheat”
The same questions in other papers
in vitro · organism · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →in vitro · yield · Triticum aestivum (wheat)Positive and negative results
28 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
- no effect
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.