Abstract
In this work, a woody species [A. peregrina (L.) Speg.] was studied in order to observe the effect of ABA and GA3 at the biochemical level during the process of seed germination. Embryos incubated in sucrose solution containing ABA and/or GA3 were analyzed through SDS-PAGE to observe the mobilization pattern of storage proteins during the beginning of germination. Cotyledons isolated from seeds incubated in aqueous solutions containing ABA and/or GA3, were also analyzed through SDS-PAGE and by PAGE/Activity Gels (polyacrylamide gels copolymerized with substrate for enzymes) to observe the mobilization pattern of storage proteins and protease activity after the beginning of the germination. Results of these experiments show that ABA blocks protein mobilization by inhibiting protease activity in cotyledons. This inhibition is not sufficient to prevent germination showing that the effect of ABA on germination is not dependent on protease activity. The blockage of storage protein mobilization was also observed in embryos, but no protease activity inhibition was clearly detected. ABA was able to induce the synthesis of proteins in cotyledons but not in embryos. A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos, but this degradation was blocked by ABA and GA3. Using the same concentrations of ABA and GA3 on embryos and cotyledons, the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons. Although the effects of ABA and GA3 were not so different from those shown in the literature, the behavior of 17 kD-polypeptide contradicts these reports suggesting that specific studies should be performed.
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Claims 11
Claim · model-written summary
ABA blocked protein mobilization through protease activity inhibition in cotyledons.
- Taxon
- Anadenanthera peregrina (L.) speg
Anadenanthera peregrinat0 - Organ / stage
- cotyledonse0
- Treatment
- ABAe0
- Gene
- proteasee0
- Outcome
- protein mobilizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA blocks protein mobilization by inhibiting protease activity in cotyledons.”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
ABA-associated protease inhibition did not prevent germination.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Outcome
- not sufficient to prevent germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This inhibition is not sufficient to prevent germination”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
ABA also blocked storage protein mobilization in embryos.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- embryose0
- Treatment
- ABAe1
- Outcome
- storage protein mobilizatione0
- Direction
- blockagee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The blockage of storage protein mobilization was also observed in embryos”
t0“Anadenanthera peregrina (L.) speg”
e1“Results of these experiments show that ABA blocks protein mobilization”
Claim · model-written summary
Protease activity inhibition was not clearly detected in embryos.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Gene
- proteasee0
- Outcome
- protease activity inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no protease activity inhibition was clearly detected”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
ABA induced protein synthesis in cotyledons.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- cotyledonse0
- Treatment
- ABAe0
- Outcome
- synthesis of proteinse0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA was able to induce the synthesis of proteins in cotyledons but not in embryos.”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
ABA did not induce protein synthesis in embryos.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- embryose0
- Treatment
- ABAe0
- Outcome
- synthesis of proteinse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA was able to induce the synthesis of proteins in cotyledons but not in embryos.”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
A 17 kD polypeptide degraded within six hours in control embryos.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- embryose0
- Gene
- polypeptidee0
- Outcome
- polypeptidee0
- Value
- 17 kDe0
- Qualifier
- within 6 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A polypeptide with an approximate molecular weight of 17 kD, was degraded within 6 hours in control embryos”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
ABA blocked degradation of the 17 kD polypeptide.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Treatment
- ABAe0
- Outcome
- degradatione0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this degradation was blocked by ABA and GA3”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
GA3 blocked degradation of the 17 kD polypeptide.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Treatment
- GA3e0
- Outcome
- degradatione0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this degradation was blocked by ABA and GA3”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
GA3 counteracted ABA in embryos.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- embryose0
- Treatment
- GA3e0
- Outcome
- effect of ABAe0
- Direction
- counteractede0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons”
t0“Anadenanthera peregrina (L.) speg”
Claim · model-written summary
GA3 did not counteract ABA in cotyledons.
- Taxon
- Anadenanthera peregrina (L.) spegAnadenanthera peregrinat0
- Organ / stage
- cotyledonse0
- Treatment
- GA3e0
- Outcome
- effect of ABAe0
- Direction
- note0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effect of ABA was counteracted by GA3 in embryos, but not in cotyledons”
t0“Anadenanthera peregrina (L.) speg”