Abstract
Abstract In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h. In this medium, the toxic effect of aluminium (Al) on the plasma membrane was investigated for up to 24 h. After the addition of Al to the cell suspension, Al started to accumulate immediately in the cells, and a maximum value was observed at 9 h. Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions. Furthermore, the AI-treated cells were stained with fluorescein diacetate (FDA) as much as untreated control cells. These results suggest that the accumulation of Al does not damage the membrane. The addition of Fe(II) to the cells which had been exposed to Al for 12 h resulted in immediate lipid peroxidation and Evans blue uptake several hours later. A combination of Al and Fe(II) caused the K leakage, a...
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Claims 8
Claim · model-written summary
Aluminium accumulated in tobacco cells immediately after addition, with a maximum at 9 h.
- Taxon
- Nicotiana tabacum L.
Nicotiana tabacume0 - Treatment
- addition of Ale1
- Outcome
- accumulatee1
- Qualifier
- a maximum value was observed at 9 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e1“After the addition of Al to the cell suspension, Al started to accumulate immediately in the cells, and a maximum value was observed at 9 h”
Claim · model-written summary
Aluminium induced callose deposition in tobacco cells.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- Ale2
- Outcome
- callose depositione2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e2“Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions”
Claim · model-written summary
Aluminium did not significantly enhance Evans blue uptake in tobacco cells.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- Ale2
- Outcome
- uptake of Evans bluee2
- Direction
- did not enhance significantlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e2“Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions”
Claim · model-written summary
Aluminium did not significantly enhance lipid peroxidation in tobacco cells.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- Ale2
- Outcome
- per oxidation of lipidse2
- Direction
- did not enhance significantlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e2“Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions”
Claim · model-written summary
Aluminium did not significantly enhance potassium ion leakage from tobacco cells.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- Ale2
- Outcome
- leakage of potassium (K) ionse2
- Direction
- did not enhance significantlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e2“Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions”
Claim · model-written summary
Accumulation of aluminium does not damage the membrane of tobacco cells.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- accumulation of Ale3
- Outcome
- does not damage the membranee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e3“These results suggest that the accumulation of Al does not damage the membrane”
Claim · model-written summary
Fe(II) added to tobacco cells pre-exposed to Al for 12 h caused immediate lipid peroxidation.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- addition of Fe(II)e4
- Outcome
- lipid peroxidatione4
- Qualifier
- immediatee4
- Comparator
- exposed to Al for 12 he4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e4“The addition of Fe(II) to the cells which had been exposed to Al for 12 h resulted in immediate lipid peroxidation and Evans blue uptake several hours later”
Claim · model-written summary
Fe(II) added to tobacco cells pre-exposed to Al for 12 h caused Evans blue uptake several hours later.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume0
- Treatment
- addition of Fe(II)e4
- Outcome
- Evans blue uptakee4
- Qualifier
- several hours latere4
- Comparator
- exposed to Al for 12 he4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a simple solution containing 3 mM CaCl2 and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h”
e4“The addition of Fe(II) to the cells which had been exposed to Al for 12 h resulted in immediate lipid peroxidation and Evans blue uptake several hours later”
The same questions in other papers
aluminium atom · nutrient: content · Nicotiana tabacum (tobacco)Results agree
7 papers in the same direction as this paper
- negative
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.