Abstract
Protein databases constructed using high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were used to explore the proteome expressed in various rice tissues. Proteins from leaf sheath, root, and cultured suspension cells were systematically analyzed using 2D-PAGE, mass spectrometry and Edman sequencing, followed by database searching. In all, 79 of the 431 spots detected by 2D-PAGE in the leaf sheath, 73 of the 508 spots in the root and 140 of the 962 spots in the cultured suspension cells could be identified. Protein lists were constructed for each tissue and used to investigate the effects of gibberellin (GA) treatment. In the leaf sheath, root and cultured suspension cells, 8, 21, and 14 of the identified proteins, respectively, were regulated by GA. These proteins included polypeptides involved in general metabolism, energy production, transcriptional regulation and signal transduction in the leaf sheath; in metabolism and defense in the root; and in metabolism, energy production, cell growth, defense and signal transduction in the cultured suspension cells. These results indicate that the proteome databases assembled in these studies will be useful for the rapid assessment of changes in protein content in specific tissues, and that proteins regulated by GA may play a significant role in tissue growth.
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Claims 12
Claim · model-written summary
In the leaf sheath, 79 of the 431 2D-PAGE spots were identified.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- leaf sheathe1
- Outcome
- spots detected by 2D-PAGEe1
- Value
- 79 of the 431 spotse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e1“In all, 79 of the 431 spots detected by 2D-PAGE in the leaf sheath, 73 of the 508 spots in the root and 140 of the 962 spots in the cultured suspension cells could be identified”
Claim · model-written summary
In the root, 73 of the 508 2D-PAGE spots were identified.
- Taxon
- riceOryza sativae0
- Organ / stage
- roote1
- Outcome
- spots detected by 2D-PAGEe1
- Value
- 73 of the 508 spotse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e1“In all, 79 of the 431 spots detected by 2D-PAGE in the leaf sheath, 73 of the 508 spots in the root and 140 of the 962 spots in the cultured suspension cells could be identified”
Claim · model-written summary
In cultured suspension cells, 140 of the 962 2D-PAGE spots were identified.
- Taxon
- riceOryza sativae0
- Organ / stage
- cultured suspension cellse1
- Outcome
- spots detected by 2D-PAGEe1
- Value
- 140 of the 962 spotse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e1“In all, 79 of the 431 spots detected by 2D-PAGE in the leaf sheath, 73 of the 508 spots in the root and 140 of the 962 spots in the cultured suspension cells could be identified”
Claim · model-written summary
Gibberellin treatment regulated 8 identified proteins in the leaf sheath.
- Taxon
- riceOryza sativae0
- Organ / stage
- leaf sheathe3
- Treatment
- gibberellin (GA) treatmente2
- Outcome
- identified proteinse3
- Value
- 8e3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e2“used to investigate the effects of gibberellin (GA) treatment”
e3“In the leaf sheath, root and cultured suspension cells, 8, 21, and 14 of the identified proteins, respectively, were regulated by GA”
Claim · model-written summary
Gibberellin treatment regulated 21 identified proteins in the root.
- Taxon
- riceOryza sativae0
- Organ / stage
- roote3
- Treatment
- gibberellin (GA) treatmente2
- Outcome
- identified proteinse3
- Value
- 21e3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e2“used to investigate the effects of gibberellin (GA) treatment”
e3“In the leaf sheath, root and cultured suspension cells, 8, 21, and 14 of the identified proteins, respectively, were regulated by GA”
Claim · model-written summary
Gibberellin treatment regulated 14 identified proteins in cultured suspension cells.
- Taxon
- riceOryza sativae0
- Organ / stage
- cultured suspension cellse3
- Treatment
- gibberellin (GA) treatmente2
- Outcome
- identified proteinse3
- Value
- 14e3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e2“used to investigate the effects of gibberellin (GA) treatment”
e3“In the leaf sheath, root and cultured suspension cells, 8, 21, and 14 of the identified proteins, respectively, were regulated by GA”
Claim · model-written summary
The gibberellin-regulated leaf sheath proteins included general metabolism, energy production and transcriptional regulation proteins.
- Taxon
- riceOryza sativae0
- Organ / stage
- leaf sheathe4
- Outcome
- general metabolism, energy production, transcriptional regulatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e4“These proteins included polypeptides involved in general metabolism, energy production, transcriptional regulation and signal transduction in the leaf sheath”
Claim · model-written summary
The gibberellin-regulated leaf sheath proteins included signal transduction proteins.
- Taxon
- riceOryza sativae0
- Organ / stage
- leaf sheathe4
- Outcome
- signal transductione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e4“These proteins included polypeptides involved in general metabolism, energy production, transcriptional regulation and signal transduction in the leaf sheath”
Claim · model-written summary
The gibberellin-regulated root proteins were involved in metabolism and defense.
- Taxon
- riceOryza sativae0
- Organ / stage
- roote5
- Outcome
- metabolism and defensee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e5“in metabolism and defense in the root”
Claim · model-written summary
The gibberellin-regulated cultured suspension cell proteins were involved in metabolism, energy production and cell growth.
- Taxon
- riceOryza sativae0
- Organ / stage
- cultured suspension cellse6
- Outcome
- metabolism, energy production, cell growthe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e6“in metabolism, energy production, cell growth, defense and signal transduction in the cultured suspension cells”
Claim · model-written summary
The gibberellin-regulated cultured suspension cell proteins were involved in defense and signal transduction.
- Taxon
- riceOryza sativae0
- Organ / stage
- cultured suspension cellse6
- Outcome
- defense and signal transductione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e6“in metabolism, energy production, cell growth, defense and signal transduction in the cultured suspension cells”
Claim · model-written summary
Proteins regulated by gibberellin may play a significant role in rice tissue growth.
- Taxon
- riceOryza sativae0
- Compound
- GAe7
- Outcome
- tissue growthe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteome expressed in various rice tissues”
e7“proteins regulated by GA may play a significant role in tissue growth”