Abstract
Twenty pepper cultivars selected from those commonly traded and cultivated in Italy were subjected to field and laboratory identification and characterization tests. In a field trial plants were grown until fruit ripening and the main morpho-physiological characters used for distinguishing pepper cultivars were recorded from the seedling stage to plant maturity. The morpho-physiological differences observed in the field allowed the clear identification of cultivars of the same group. In the laboratory, ultrathin-layer isoelectrofocusing (UTLIEF) and SDS capillary gel electrophoresis (SDS-CGE) of the seed proteins, urea-detergent soluble and water-soluble respectively, were performed. The high resolving power of UTLIEF was suitable for detecting differences between pepper cultivars and together with densitometer analysis offered considerable potential for the characterization of pepper cultivars. The correlation with some morpho physiological characters and the advantage of providing excellent resolution in a short time indicated the possible use of UTLIEF of urea-detergent proteins as a standard identification method for pepper cultivars. Despite the low variability of the water-soluble proteins analyzed, four different electrophoregrams were detected by SDS-CGE for the twenty cultivars. The resolution and efficiency of separation confirmed SDS-CGE as a valid and complementary tool to gel electrophoresis for cultivar identification.
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Claims 6
Claim · model-written summary
Field morphology clearly identified cultivars in the same group.
Verbatim
e0“The morpho-physiological differences observed in the field allowed the clear identification of cultivars of the same group.”
t0“pepper (Capsicum spp.)”
Claim · model-written summary
UTLIEF resolved differences between pepper cultivars.
Verbatim
e0“The high resolving power of UTLIEF was suitable for detecting differences between pepper cultivars”
Claim · model-written summary
UTLIEF combined with densitometry had potential for pepper cultivar characterization.
Verbatim
e0“together with densitometer analysis offered considerable potential for the characterization of pepper cultivars”
Claim · model-written summary
UTLIEF of urea-detergent proteins was proposed as a standard pepper identification method.
Verbatim
e0“indicated the possible use of UTLIEF of urea-detergent proteins as a standard identification method for pepper cultivars.”
Claim · model-written summary
SDS-CGE detected four electrophoregram types among twenty cultivars despite low water-soluble protein variability.
Verbatim
e0“Despite the low variability of the water-soluble proteins analyzed, four different electrophoregrams were detected by SDS-CGE for the twenty cultivars.”
t0“pepper (Capsicum spp.)”
Claim · model-written summary
SDS-CGE was judged a valid complementary identification tool.
Verbatim
e0“The resolution and efficiency of separation confirmed SDS-CGE as a valid and complementary tool to gel electrophoresis for cultivar identification.”
t0“pepper (Capsicum spp.)”