Abstract
The rooting parameters of shoot parts of hybrid hazelnut(Corylus heterophylla × C.avellana 'Dawei') were investigated,and the activities of IAA oxidase(IAAO),polyphenoloxidase(PPO) and peroxidase(POD) in phloem at various rooting phase in different shoot parts were measured,in a simple random replication experiment.The growing shoot were cut into three sections: top part,middle part and bottom part,then treated with IBA at a concentration of 1 500 mg·L-1with a water dipping as control.The root formation position was primarily at the phloem.Rooting timing of different shoot parts was different,younger terminal portions needed 13 d to form adventitious root,middle portions and basal portions needed 19 d and 22 d,respectively.The current season's growth treated with IBA at a concentration of 1 500 mg·L-1 rooted well,the rooting percentage,root numbers and the length of root were significantly greater than that of the control ones.The terminal portions from the growing shoot rooted best,while the rooting ability of suckers terminal portions,middle portions and basal portions from the growing shoot were in the order of greatness to smallness.After treatment arrangement,the activity of POD showed an increasing pattern,with a peak at the formation of the first regeneration root.Between the treatments and the controls,different shoot parts expressed the related peaks,and magnitude of peaks was significantly positive correlated with the rooting percentage.During the cutting duration,the activity of PPO showed a double peak pattern,with the first increase at the time from starting to callus formation,and the second one from the induction phase to the first regeneration root forming,and the highest peak at root formation.Afterwards,the peak became lower.During rooting process,there were differences between the different shoot parts,and between the treatments and the controls,the activity of PPO had a difference.The activity of IAAO reached to peak at the induction phase between the callus formation time and the first regeneration root formation.
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Claims 11
Claim · model-written summary
Roots formed primarily at the phloem.
Verbatim
e0“The root formation position was primarily at the phloem.”
t0“hybird hazelnut”
Claim · model-written summary
Terminal cuttings formed adventitious roots in 13 days.
Verbatim
t0“hybird hazelnut”
e0“younger terminal portions needed 13 d to form adventitious root”
Claim · model-written summary
Middle cuttings formed adventitious roots in 19 days.
Verbatim
t0“hybird hazelnut”
e0“middle portions and basal portions needed 19 d and 22 d,respectively”
Claim · model-written summary
Basal cuttings formed adventitious roots in 22 days.
Verbatim
t0“hybird hazelnut”
e0“middle portions and basal portions needed 19 d and 22 d,respectively”
Claim · model-written summary
IBA increased rooting percentage, root number and root length significantly.
Verbatim
e0“The current season's growth treated with IBA at a concentration of 1 500 mg·L-1 rooted well,the rooting percentage,root numbers and the length of root were significantly greater than that of the control ones.”
t0“hybird hazelnut”
Claim · model-written summary
Terminal shoot portions rooted best.
Verbatim
t0“hybird hazelnut”
e0“The terminal portions from the growing shoot rooted best”
Claim · model-written summary
POD activity increased and peaked at first regenerated root formation.
Verbatim
t0“hybird hazelnut”
e0“the activity of POD showed an increasing pattern,with a peak at the formation of the first regeneration root”
Claim · model-written summary
POD peak magnitude positively correlated with rooting percentage.
Verbatim
t0“hybird hazelnut”
e0“magnitude of peaks was significantly positive correlated with the rooting percentage”
e1“the activity of POD showed an increasing pattern,with a peak at the formation of the first regeneration root”
Claim · model-written summary
PPO activity had two peaks, with the highest at root formation.
Verbatim
t0“hybird hazelnut”
e0“the activity of PPO showed a double peak pattern,with the first increase at the time from starting to callus formation,and the second one from the induction phase to the first regeneration root forming,and the highest peak at root formation”
Claim · model-written summary
PPO activity differed across shoot parts and treatments.
Verbatim
t0“hybird hazelnut”
e0“between the different shoot parts,and between the treatments and the controls,the activity of PPO had a difference”
Claim · model-written summary
IAAO activity peaked during induction between callus and first root formation.
Verbatim
t0“hybird hazelnut”
e0“The activity of IAAO reached to peak at the induction phase between the callus formation time and the first regeneration root formation.”