Abstract
Cowpea (Vigna unguiculata) is a warm-season legume that is primarily cultivated for protein rich grain. Seed size is an important breeding target that distinguishes most domesticated crops from their wild relatives and is a particularly important trait for the grain legumes. This dissertation describes efforts to breed cowpea varieties with larger seeds using marker-assisted approaches to breeding. The first chapter of this dissertation describes the development of a consensus genetic map of 1,107 molecular markers which was constructed by analyzing bead-assay genotype data from 13 experimental populations. The content and organization of the cowpea genome was also compared to the genome of soybean (Glycine max) to describe regions of synteny. The second chapter utilizes the genetic map, legume synteny, and phenotypic information collected from field and greenhouse trials to develop associations between allelic variation and the inheritance of seed size. Many of the regions of the cowpea genome important for seed size were found to be syntenic with regions of the soybean genome that were previously associated with the inheritance of seed size. These marker-trait associations are applied in the third chapter to breed cowpea varieties with up to 52% larger seeds which was accomplished by introgressing a rare Mozambican haplotype into the genetic background of a California blackeyed pea. Preliminary field screening identified introgression lines that also performed well for other important agronomic traits including yield, maturity, and plant architecture. The introgression lines developed in this work could be used as parents for deploying large seed size in other pedigrees and could be studied to better understand the impact of seed size on nutritional content and agronomic performance.
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Claims 7
Claim · model-written summary
A consensus cowpea genetic map contained 1,107 molecular markers from 13 experimental populations.
- Taxon
- cowpea
Vigna unguiculatae1 - Outcome
- consensus genetic mape0
- Value
- 1,107 molecular markerse0
- Qualifier
- 13 experimental populationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a consensus genetic map of 1,107 molecular markers which was constructed by analyzing bead-assay genotype data from 13 experimental populations”
e1“The content and organization of the cowpea genome”
Claim · model-written summary
The cowpea and soybean genomes had regions of synteny.
- Taxon
- cowpeaVigna unguiculatae0
- Outcome
- regions of syntenye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The content and organization of the cowpea genome was also compared to the genome of soybean (Glycine max) to describe regions of synteny”
Claim · model-written summary
Allelic variation was associated with inheritance of cowpea seed size.
- Taxon
- cowpeaVigna unguiculatae1
- Gene
- allelic variatione0
- Outcome
- inheritance of seed sizee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“develop associations between allelic variation and the inheritance of seed size”
e1“This dissertation describes efforts to breed cowpea varieties with larger seeds using marker-assisted approaches to breeding”
Claim · model-written summary
Many cowpea genomic regions important for seed size were syntenic with soybean seed-size regions.
- Taxon
- cowpeaVigna unguiculatae0
- Outcome
- syntenic with regions of the soybean genomee0
- Qualifier
- important for seed sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many of the regions of the cowpea genome important for seed size were found to be syntenic with regions of the soybean genome that were previously associated with the inheritance of seed size”
Claim · model-written summary
Introgression of a rare Mozambican haplotype produced cowpea varieties with up to 52% larger seeds in a California blackeyed pea background.
- Taxon
- cowpeaVigna unguiculatae0
- Material
- rare Mozambican haplotypee0
- Treatment
- introgressing a rare Mozambican haplotypee0
- Outcome
- seedse0
- Direction
- largere0
- Value
- up to 52%e0
- Comparator
- California blackeyed peae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“breed cowpea varieties with up to 52% larger seeds which was accomplished by introgressing a rare Mozambican haplotype into the genetic background of a California blackeyed pea”
Claim · model-written summary
Preliminary field screening found introgression lines that performed well for yield, maturity, and plant architecture.
- Taxon
- cowpeaVigna unguiculatae1
- Material
- introgression linese0
- Outcome
- yield, maturity, and plant architecturee0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preliminary field screening identified introgression lines that also performed well for other important agronomic traits including yield, maturity, and plant architecture”
e1“This dissertation describes efforts to breed cowpea varieties with larger seeds using marker-assisted approaches to breeding”
Claim · model-written summary
The authors suggest using the introgression lines as parents to deploy large seed size in other pedigrees.
- Taxon
- cowpeaVigna unguiculatae1
- Material
- introgression linese0
- Outcome
- used as parents for deploying large seed size in other pedigreese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The introgression lines developed in this work could be used as parents for deploying large seed size in other pedigrees”
e1“This dissertation describes efforts to breed cowpea varieties with larger seeds using marker-assisted approaches to breeding”
The same questions in other papers
transgenic · yield · Vigna unguiculata (cowpea)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.