By organism › Bacteria › Xanthobacteraceae › Azorhizobium caulinodans

Observationobservedoriginal resultmeasured

Claim · model-written summary

AcfR has a phosphorylatable REC domain and a LuxR DNA binding domain.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse0
Gene: AcfRe0
Outcome: phosphorylatable N-terminal REC (receiver) domaine0

Verbatim

e0
“AcfR, with a phosphorylatable N-terminal REC (receiver) domain and a C-terminal HTH (helix-turn-helix) LuxR DNA-binding domain in A. caulinodans ORS571”
✓ located verbatim in the source · abstract, characters 774–925

Title

FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Wei Liu; Xue Bai; Yan Li; et al. BMC Microbiology 2021. doi:10.1186/s12866-021-02138-w

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

AcfR is phylogenetically close to NarL/FixJ regulators.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse1
Gene: AcfRe0
Outcome: close evolutionary relationships with NarL/FixJ family regulatorse0

Verbatim

e0
“Phylogenetic analysis showed that AcfR possessed close evolutionary relationships with NarL/FixJ family regulators”
✓ located verbatim in the source · abstract, characters 927–1041

e1
“in A. caulinodans ORS571”
✓ located verbatim in the source · abstract, characters 901–925

Title

FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Wei Liu; Xue Bai; Yan Li; et al. BMC Microbiology 2021. doi:10.1186/s12866-021-02138-w

Other claims from this paper (6)

Observationobservedoriginal resultsimulated

Claim · model-written summary

Six histidine kinases were predicted to interact with AcfR.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse1
Gene: AcfRe0
Outcome: predicted to interact with AcfRe0
Value: six histidine kinasese0

Verbatim

e0
“six histidine kinases containing HATPase_c and HisKA domains were predicted to interact with AcfR”
✓ located verbatim in the source · abstract, characters 1056–1153

e1
“in A. caulinodans ORS571”
✓ located verbatim in the source · abstract, characters 901–925

Title

FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Wei Liu; Xue Bai; Yan Li; et al. BMC Microbiology 2021. doi:10.1186/s12866-021-02138-w

Other claims from this paper (6)

Observationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

The ΔacfR mutant had significantly reduced free-living motility.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse1
Material: ΔacfR mutante0
Outcome: cell motility behavioure0
Direction: reducede0
Comparator: wild-type straine0

Verbatim

e0
“In the free-living state, the cell motility behaviour and exopolysaccharide production of the ΔacfR mutant were significantly reduced compared to those of the wild-type strain”
✓ located verbatim in the source · abstract, characters 1318–1493

e1
“in A. caulinodans ORS571”
✓ located verbatim in the source · abstract, characters 901–925

Title

FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Wei Liu; Xue Bai; Yan Li; et al. BMC Microbiology 2021. doi:10.1186/s12866-021-02138-w

Other claims from this paper (6)

Observationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

The ΔacfR mutant had significantly reduced free-living exopolysaccharide production.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse1
Material: ΔacfR mutante0
Outcome: exopolysaccharide productione0
Direction: reducede0
Comparator: wild-type straine0

Verbatim

e0
“In the free-living state, the cell motility behaviour and exopolysaccharide production of the ΔacfR mutant were significantly reduced compared to those of the wild-type strain”
✓ located verbatim in the source · abstract, characters 1318–1493

e1
“in A. caulinodans ORS571”
✓ located verbatim in the source · abstract, characters 901–925

Title

FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Wei Liu; Xue Bai; Yan Li; et al. BMC Microbiology 2021. doi:10.1186/s12866-021-02138-w

Other claims from this paper (6)

Observation AcfR has a phosphorylatable REC domain and a LuxR DNA binding domain.
Observation AcfR is phylogenetically close to NarL/FixJ regulators.
Observation Six histidine kinases were predicted to interact with AcfR.
Observation The ΔacfR mutant had significantly reduced free-living motility.
Observation The ΔacfR mutant was competitively defective in nodulation of host stems and roots.
Mechanism AcfR is interpreted to give A. caulinodans competitive ability for symbiotic nodulation.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sequence analysis of the A. caulinodans ORS571 hydrogenase gene cluster revealed 20 open reading frames.

Taxon: Azorhizobium caulinodans ORS571Azorhizobium caulinodanst0
Outcome: 20 open reading framese0

Verbatim

t0
“Azorhizobium caulinodans ORS571”
✓ located verbatim in the source · title, characters 49–80

e0
“Sequence analysis revealed the presence of 20 open reading frames”
✓ located verbatim in the source · abstract, characters 116–181

Title

Molecular and functional characterization of the Azorhizobium caulinodans ORS571 hydrogenase gene cluster

Cecilia Baginsky; Jose-Manuel Palacios; Juan Imperial; et al. FEMS Microbiology Letters 2004. doi:10.1111/j.1574-6968.2004.tb09723.x

Other claims from this paper (4)

Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

The organization of the A. caulinodans ORS571 hydrogenase system suggests a close relatedness to that of Rhodobacter capsulatus.

Taxon: A. caulinodans ORS571Azorhizobium caulinodanse0
Outcome: close relatedness to that of Rhodobacter capsulatuse0

Verbatim

e0
“The physical and genetic organization of A. caulinodans ORS571 hydrogenase system suggests a close relatedness to that of Rhodobacter capsulatus”
✓ located verbatim in the source · abstract, characters 226–370

Title

Molecular and functional characterization of the Azorhizobium caulinodans ORS571 hydrogenase gene cluster

Cecilia Baginsky; Jose-Manuel Palacios; Juan Imperial; et al. FEMS Microbiology Letters 2004. doi:10.1111/j.1574-6968.2004.tb09723.x

Other claims from this paper (4)

Observationobservedoriginal resultmeasured

Claim · model-written summary

A gene homologous to Rhizobium leguminosarum hupE was identified downstream of the hyp operon in A. caulinodans.

Taxon: Azorhizobium caulinodans ORS571Azorhizobium caulinodanst0
Gene: hupEe0
Outcome: gene homologous to Rhizobium leguminosarum hupE was identifiede0

Verbatim

t0
“Azorhizobium caulinodans ORS571”
✓ located verbatim in the source · title, characters 49–80

e0
“a gene homologous to Rhizobium leguminosarum hupE was identified downstream of the hyp operon”
✓ located verbatim in the source · abstract, characters 407–500

Title

Molecular and functional characterization of the Azorhizobium caulinodans ORS571 hydrogenase gene cluster

Cecilia Baginsky; Jose-Manuel Palacios; Juan Imperial; et al. FEMS Microbiology Letters 2004. doi:10.1111/j.1574-6968.2004.tb09723.x

Other claims from this paper (4)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Both bacterial oxidase mutations were null alleles.

Taxon: Azorhizobium caulinodansAzorhizobium caulinodanst0
Gene: cytbd- and cytcbb3-e0
Outcome: null allelese0

Verbatim

e0
“As evidenced by visible absorbance spectra taken from mutant bacteria carrying perfect gene replacements, both the cytbd- and cytcbb3- mutations were null alleles.”
✓ located verbatim in the source · abstract, characters 321–484

t0
“Azorhizobium caulinodans”
✓ located verbatim in the source · title, characters 0–24

Title

Azorhizobium caulinodans uses both cytochrome bd (quinol) and cytochrome cbb3 (cytochrome c) terminal oxidases for symbiotic N2 fixation

P A Kaminski; C L Kitts; Z Zimmerman; et al. Journal of Bacteriology 1996. doi:10.1128/jb.178.20.5989-5994.1996

Other claims from this paper (11)

Intervention effect The cytbd single mutant fixed nitrogen at 70 to 90% of wild-type rates under 2% oxygen.
Intervention effect The cytcbb3 single mutant fixed nitrogen at 70 to 90% of wild-type rates under 2% oxygen.
Intervention effect Double mutants completely lacked symbiotic nitrogen fixation.
Mechanism Both oxidases drive respiration during symbiotic nitrogen fixation at nanomolar oxygen.
Intervention effect Double mutants fixed nitrogen at 70% of wild-type rates in culture under 2% oxygen.
Mechanism Residual nitrogen fixation presumably reflects other terminal oxidase activities.
Observation cytbd oxidase maintained dissolved oxygen at 3.6 microM.
Observation cytcbb3 oxidase depleted oxygen to submicromolar levels.
Mechanism cytcbb3 activity disproportionately supported microaerobic growth.
Observation cytbd oxidase was inactive below 3.6 microM dissolved oxygen in oxygen-limited culture.
Mechanism Both oxidases seem to contribute equally in Sesbania nodules at 10 to 20 nM oxygen.

Observationobservedoriginal resultmeasured

Claim · model-written summary

cytcbb3 oxidase depleted oxygen to submicromolar levels.

Taxon: Azorhizobium caulinodansAzorhizobium caulinodanst0
Gene: cytcbb3 oxidasee0
Outcome: O2e0
Direction: depletede0
Value: submicromolar levelse0

Verbatim

e0
“cytcbb3 oxidase activity depleted O2 to submicromolar levels.”
✓ located verbatim in the source · abstract, characters 1441–1502

t0
“Azorhizobium caulinodans”
✓ located verbatim in the source · title, characters 0–24

Title

Azorhizobium caulinodans uses both cytochrome bd (quinol) and cytochrome cbb3 (cytochrome c) terminal oxidases for symbiotic N2 fixation

P A Kaminski; C L Kitts; Z Zimmerman; et al. Journal of Bacteriology 1996. doi:10.1128/jb.178.20.5989-5994.1996

Other claims from this paper (11)

Observation Both bacterial oxidase mutations were null alleles.
Intervention effect The cytbd single mutant fixed nitrogen at 70 to 90% of wild-type rates under 2% oxygen.
Intervention effect The cytcbb3 single mutant fixed nitrogen at 70 to 90% of wild-type rates under 2% oxygen.
Intervention effect Double mutants completely lacked symbiotic nitrogen fixation.
Mechanism Both oxidases drive respiration during symbiotic nitrogen fixation at nanomolar oxygen.
Intervention effect Double mutants fixed nitrogen at 70% of wild-type rates in culture under 2% oxygen.
Mechanism Residual nitrogen fixation presumably reflects other terminal oxidase activities.
Observation cytbd oxidase maintained dissolved oxygen at 3.6 microM.
Mechanism cytcbb3 activity disproportionately supported microaerobic growth.
Observation cytbd oxidase was inactive below 3.6 microM dissolved oxygen in oxygen-limited culture.
Mechanism Both oxidases seem to contribute equally in Sesbania nodules at 10 to 20 nM oxygen.

Azorhizobium caulinodans 33 claims

33 claims